Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture

Methods Mol Biol. 2018:1815:89-101. doi: 10.1007/978-1-4939-8594-4_5.

Abstract

Scientific photography is an important and indispensable tool in plant tissue culture research: photographs should be taken throughout a project for documentation. The aim of photography in plant tissue culture should be to illustrate clearly the differentiation, growth, and developmental stages occurring in vitro. Poor-quality scientific photography in tissue culture research and professional reports results in poor documentation. If visual aspects of the tissue culture are not well documented or not well reproduced in the image, an important part of the research is missed, the resulting report is of limited scientific value, and the research results may not be reproducible. Simple methods for improving the results of photography of materials from plant tissue culture are described and discussed, along with the necessary photographic equipment, suitable backgrounds, the construction of photographic plates, and correct use of electronic files for images. Finally, ethical concerns about image manipulation are discussed.

Keywords: Digital photography; Ethical considerations; Image manipulation; Image processing; Image storage; Photography; Plant development; Plant tissue culture.

MeSH terms

  • Image Processing, Computer-Assisted
  • Microscopy
  • Photography / methods*
  • Research*
  • Tissue Culture Techniques / methods*