A transcriptome study on Macrobrachium nipponense hepatopancreas experimentally challenged with white spot syndrome virus (WSSV)

PLoS One. 2018 Jul 6;13(7):e0200222. doi: 10.1371/journal.pone.0200222. eCollection 2018.

Abstract

White spot syndrome virus (WSSV) is one of the most devastating pathogens of cultured shrimp, responsible for massive loss of its commercial products worldwide. The oriental river prawn Macrobrachium nipponense is an economically important species that is widely farmed in China and adult prawns can be infected by WSSV. However, the molecular mechanisms of the host pathogen interaction remain unknown. There is an urgent need to learn the host pathogen interaction between M. nipponense and WSSV which will be able to offer a solution in controlling the spread of WSSV. Next Generation Sequencing (NGS) was used in this study to determin the transcriptome differences by the comparison of control and WSSV-challenged moribund samples, control and WSSV-challenged survived samples of hepatopancreas in M. nipponense. A total of 64,049 predicted unigenes were obtained and classified into 63 functional groups. Approximately, 4,311 differential expression genes were identified with 3,308 genes were up-regulated when comparing the survived samples with the control. In the comparison of moribund samples with control, 1,960 differential expression genes were identified with 764 genes were up-regulated. In the contrast of two comparison libraries, 300 mutual DEGs with 95 up-regulated genes and 205 down-regulated genes. All the DEGs were performed GO and KEGG analysis, overall a total of 85 immune-related genes were obtained and these gene were groups into 13 functions and 4 KEGG pathways, such as protease inhibitors, heat shock proteins, oxidative stress, pathogen recognition immune receptors, PI3K/AKT/mTOR pathway, MAPK signaling pathway and Ubiquitin Proteasome Pathway. Ten genes that valuable in immune responses against WSSV were selected from those DEGs to furture discuss the response of host to WSSV. Results from this study contribute to a better understanding of the immune response of M. nipponense to WSSV, provide information for identifying novel genes in the absence of genome of M. nipponense. Furthermore, large number of transcripts obtained from this study could provide a strong basis for future genomic research on M. nipponense.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arthropod Proteins / genetics*
  • Arthropod Proteins / metabolism
  • Gene Expression Regulation*
  • Hepatopancreas / metabolism*
  • High-Throughput Nucleotide Sequencing
  • Palaemonidae / genetics*
  • Palaemonidae / metabolism
  • Palaemonidae / virology
  • Signal Transduction / genetics
  • Transcriptome*
  • White spot syndrome virus 1*

Substances

  • Arthropod Proteins

Grants and funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31572617), the New Cultivar Breeding Major Project in Jiangsu Province (PZCZ201745), the Science & Technology Supporting Program of Jiangsu Province (BE2016308), the China Agriculture Research System-48(CARS-48), the Central Public-interest Scientific Institution Basal Research Fund CAFS (2017JBFZ05), the Science and Technology Development Fund of Wuxi (CLE02N1514). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.