The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress

Yuying Qi; Yanan Liu; Zixin Zhang; Jiaojiao Gao; Zhiyong Guan; Weimin Fang; Sumei Chen; Fadi Chen; Jiafu Jiang

doi:10.1038/s41438-018-0037-y

The over-expression of a chrysanthemum gene encoding an RNA polymerase II CTD phosphatase-like 1 enzyme enhances tolerance to heat stress

Hortic Res. 2018 Jul 1:5:37. doi: 10.1038/s41438-018-0037-y. eCollection 2018.

Authors

Yuying Qi¹, Yanan Liu¹, Zixin Zhang¹, Jiaojiao Gao¹, Zhiyong Guan¹, Weimin Fang¹, Sumei Chen¹, Fadi Chen¹, Jiafu Jiang¹

Affiliation

¹ College of Horticulture, Key Laboratory of Landscaping, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.

Abstract

The enzyme RNAPII CTD phosphatase-like 1 is known as a transcriptional regulator of the plant response to various abiotic stresses. Here, the isolation of CmCPL1, a chrysanthemum (Chrysanthemum morifolium) gene encoding this enzyme is described. Its predicted 955 residue gene product includes the FCPH catalytic domain, two double-stranded RNA binding motifs, and a nuclear localization signal. A sub-cellular localization assay confirmed that CmCPL1 was expressed in the nucleus. CmCPL1 transcription was shown to be significantly inducible by heat stress. The over-expression and knockdown of CmCPL1, respectively, increased and diminished the tolerance of chrysanthemum to heat stress, which maybe dependent on the regulation of CmCPL1 and on the expression of downstream heat stress-responsive genes.