Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS

Chem Commun (Camb). 2018 Aug 21;54(65):8972-8975. doi: 10.1039/c8cc04437f. Epub 2018 Jul 5.

Abstract

Fast (>100 kHz) magic angle spinning solid-state NMR allows combining high-sensitive proton detection with the absence of an intrinsic molecular weight limit. Using this technique we observe for the first time narrow 1H RNA resonances and assign nucleotide spin systems with only 200 μg of uniformly 13C,15N-labelled RNA.

MeSH terms

  • Archaeal Proteins / chemistry*
  • Carbon Isotopes
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protons*
  • Pyrococcus furiosus / chemistry
  • RNA / chemistry*
  • RNA-Binding Proteins / chemistry*

Substances

  • Archaeal Proteins
  • Carbon Isotopes
  • Nitrogen Isotopes
  • Protons
  • RNA-Binding Proteins
  • RNA