DNA Organization and Superesolved Segregation

Diego I Cattoni; Jean-Bernard Fiche; Antoine Le Gall; Marcelo Nollmann

doi:10.1007/978-1-4939-8556-2_14

DNA Organization and Superesolved Segregation

Methods Mol Biol. 2018:1805:271-289. doi: 10.1007/978-1-4939-8556-2_14.

Authors

Diego I Cattoni¹, Jean-Bernard Fiche¹, Antoine Le Gall¹, Marcelo Nollmann²

Affiliations

¹ Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, Montpellier, France.
² Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, Montpellier, France. marcelo.nollmann@cbs.cnrs.fr.

PMID: 29971723
DOI: 10.1007/978-1-4939-8556-2_14

Abstract

With single-molecule localization microscopy (SMLM) it is possible to reveal the internal composition, architecture, and dynamics of molecular machines and large cellular complexes. SMLM remains technically challenging, and frequently its implementation requires tailored experimental conditions that depend on the complexity of the subcellular structure of interest. Here, we describe two simple, robust, and high-throughput protocols to study molecular motors and machineries responsible for chromosome transport and organization in bacteria using 2D- and 3D-SMLM.

Keywords: Bacillus subtilis; Bacterial chromosome; Molecular motors; ParABS; Photoactivated localization microscopy (PALM); SpoIIIE; Sporulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / metabolism*
DNA Replication
DNA, Bacterial / metabolism*
Imaging, Three-Dimensional
Microfluidics
Spores, Bacterial / metabolism

Substances

DNA, Bacterial