Employing high-throughput sequencing as the method, this study analyzed the relationship between the water environment and the microbial community structure in the surface-flow constructed wetland. The results showed that: the mean Shannon-Wiener index was 6.57 and there were mainly 18 categories in the microbial community, including Proteobacterice (38.97%), Bacteroidetes (15.63%) etc. Of these, the total content of 22 genera was over 1%. The microbial biodiversity showed an increasing trend at the beginning and then turned to a decreasing trend in the flowing direction. The results also revealed that pH, ORP, NH4+-N, NO2--N and TN acted as important restricting factors for the microbial community.
Keywords: high-throughput sequencing; microbial community; nitrogen; surface-flow constructed wetlands; water environment.