Genetic variation at the long noncoding RNA H19 gene is associated with the risk of hypertrophic cardiomyopathy

Epigenomics. 2018 Jul;10(7):865-873. doi: 10.2217/epi-2017-0175. Epub 2018 Jul 2.

Abstract

Aim: The long noncoding RNA H19 and its host micro RNA miR-675 have been found deregulated in cardiac hypertrophy and heart failure tissues. Our aim was to investigate whether the H19 gene variants were associated with the risk of hypertrophic cardiomyopathy (HCM).

Patients & methods: We genotyped two H19 tag single nucleotide polymorphisms in 405 HCM patients and 550 controls, and sequenced this gene in 100 patients.

Results: The rs2107425 C was significantly increased in sarcomere no-mutation patients (n = 225; p = 0.01): CC versus CT + TT, p = 0.017; odd ratios: 1.51. Sequencing of the H19 coding transcript identified two patients heterozygous carriers for a rare variant, rs945977096 G/A, that was absent among the controls.

Conclusion: Our study suggested a significant association between H19 variants and the risk of developing HCM.

Keywords: H19 gene; gene polymorphism; hypertrophic cardiomyopathy; long-noncoding RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Cardiomyopathy, Hypertrophic / genetics*
  • Case-Control Studies
  • Female
  • Genetic Predisposition to Disease*
  • Genotyping Techniques / methods
  • Humans
  • Male
  • Middle Aged
  • Mutation
  • Polymorphism, Single Nucleotide
  • RNA, Long Noncoding / genetics*
  • Risk Factors
  • Sequence Analysis, DNA
  • Young Adult

Substances

  • H19 long non-coding RNA
  • RNA, Long Noncoding