High-Resolution and High-Speed Atomic Force Microscope Imaging

Francesca Zuttion; Lorena Redondo-Morata; Arin Marchesi; Ignacio Casuso

doi:10.1007/978-1-4939-8591-3_11

High-Resolution and High-Speed Atomic Force Microscope Imaging

Methods Mol Biol. 2018:1814:181-200. doi: 10.1007/978-1-4939-8591-3_11.

Authors

Francesca Zuttion¹, Lorena Redondo-Morata², Arin Marchesi¹, Ignacio Casuso³

Affiliations

¹ LAI, Aix-Marseille Université, INSERM UMR_S 1067, CNRS UMR 7333, 13009, Marseille, France.
² Inserm U1019, Institut Pasteur de Lille, Center for Infection and Immunity of Lille, Lille, France.
³ LAI, Aix-Marseille Université, INSERM UMR_S 1067, CNRS UMR 7333, 13009, Marseille, France. ignacio.casuso@inserm.fr.

PMID: 29956233
DOI: 10.1007/978-1-4939-8591-3_11

Abstract

The advent of high-speed atomic force microscopy (HS-AFM) over the recent years has opened up new horizons for the study of structure, function and dynamics of biological molecules. HS-AFM is capable of 1000 times faster imaging than conventional AFM. This circumstance uniquely enables the observation of the dynamics of all the molecules present in the imaging area. Over the last 10 years, the HS-AFM has gone from a prototype-state technology that only a few labs in the world had access to (including ours) to an established commercialized technology that is present in tens of labs around the world. In this protocol chapter we share with the readers our practical know-how on high resolution HS-AFM imaging.

Keywords: Cantilever; Electron beam deposited tip; Feedback optimization; HS-AFM; High speed atomic force microscope; Image treatment; Oscillation amplitude; PID; Plasma EBD tip sharpening; Tip–sample approach.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Imaging, Three-Dimensional*
Lipid Bilayers / chemistry
Microscopy, Atomic Force / methods*
Video Recording

Substances

Lipid Bilayers