A novel Gram-stain-negative, strictly aerobic, polar-flagellated and rod-shaped bacterium, designated SC1-8T, was isolated from a dust collector at a pig farm located in Wanju-gun, Jeollabuk-do, Republic of Korea. The strain grew within a temperature range of 4-37 °C (optimum, 28-30 °C), at pH 7.0-9.0 (pH 7.0-8.0) and with 0-2 % (w/v) NaCl (0 %). Colonies were white-beige, circular and convex after 4 days of incubation on Reasoner's 2A agar. Based on the 16S rRNA gene sequence analysis, strain SC1-8T was a member of the genus Simplicispira, revealing the highest sequence similarities to Simplicispira limi EMB325T (97.9 %), Simplicispira psychrophila DSM 11588T (97.4 %), Acidovorax defluvii BSB411T (97.3 %), Simplicispira piscis RSG39T (97.1 %) and Simplicispira metamorpha DSM 1837T (97.0 %). The predominant respiratory quinone was Q-8. The polar lipids were phosphatidylethanolamone, diphosphatidylglycerol and phosphatidylglycerol. The major fatty acids (>10 % of the total fatty acids) were composed of C16 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The DNA G+C content was 63.3 mol%. On the basis of phenotypic, genotypic and phylogenetic evidence, strain SC1-8T is presented as a novel species, for which the name Simplicispira suum sp. nov. is proposed. The type strain is SC1-8T (=KACC 19329T=NBRC 113111T).
Keywords: Simplicispira suum; dust collector; new taxa; taxonomy.