5-20 wt% trimethoxysilylpropyl octadecyldimethyl ammonium chloride (QAS) was used to modify Poly (ε-caprolactone) (PCL)-gelatin hybrid to fabricate non-leaching antibacterial nanofiber membranes (PG-Q) by electrospinning. The results from scanning electron microscopy (SEM) and transmission electron microscopy (TEM) indicated that the QAS leaded to phase separation between the QAS and PCL. Hydrophilic test demonstrated that the PG-Q nanofiber membranes had hydrophobic surface, which was help for peeling off the dressing from the wound. Additionally, the physical and chemical cross-linking between the QAS/PCL and QAS/gelatin were confirmed by Fourier transform infrared (FTIR), which were good for long lasting antibacterial effect. The PG-Q membranes also showed excellent cell-biocompatibility. Furthermore, compared with pure PCL nanofiber membrane, the PG-Q nanofiber membranes, especially PG-Q15 (QAS: 15 wt%) and PG-Q20 (QAS: 20 wt%), showed a considerable increase in the bacteriostatic rate of S. aureus and P. aeruginosa (more than 99% after 12 h). Therefore, electrospinning non-leaching antibacterial nanofiber membranes could be an optimal choice for antibacterial wound dressing.
本研究采用 5～20 wt% 的三甲氧基硅基丙基十八烷基二甲氧氯化铵（QAS）改性聚己内酯（PCL）/明胶复合材料（PG-Q），再通过静电纺丝技术制备出一种非溶出广谱抑菌型纳米纤维膜。扫描电镜和透射电镜观察表明 QAS 能促使明胶和 PCL 的相分离。动态水接触角结果证明 QAS 能够提高膜的表面憎水性，有利于敷料与伤口剥离。傅里叶变换红外光谱结果证明 QAS 与 PCL 及明胶之间存在着氢键及化学交联作用，有利于长效抑菌。此外，PG-Q 膜具有良好的细胞亲和性。其中 QAS 占 15 wt% 及 20 wt% 的样品 12 h 后对金葡菌及绿脓杆菌的抑制率均超过 99%。该 PG-Q 膜可作为一种抑菌型伤口敷料用于创面护理。.
Keywords: antibacterial; electrospinning; quaternary ammonium salts; wound dressing.