Multiplexed discrimination of microRNA single nucleotide variants through triplex molecular beacon sensors

Chem Commun (Camb). 2018 Jul 5;54(55):7673-7676. doi: 10.1039/c8cc03574a.

Abstract

Herein, we develop a new nanopore sensing strategy for the selective detection of microRNAs and single nucleotide variants (SNVs) based on triplex molecular beacon sensors. This sensing system shows very high specificity in discriminating microRNA SNVs and can be applied for the simultaneous detection of several microRNAs of the same family in a mixture.

MeSH terms

  • Biosensing Techniques / methods
  • DNA Probes / chemistry
  • DNA Probes / genetics*
  • Limit of Detection
  • Lipid Bilayers / chemistry
  • MicroRNAs / analysis*
  • MicroRNAs / genetics*
  • Nanopores
  • Nucleic Acid Hybridization
  • Phosphatidylcholines / chemistry
  • Polymorphism, Single Nucleotide / genetics*

Substances

  • DNA Probes
  • Lipid Bilayers
  • MicroRNAs
  • Phosphatidylcholines
  • 1,2-diphytanoylphosphatidylcholine