The influence of phosphorus on the autotrophic and mixotrophic denitrification

Chunzhen Fan; Peiqi Wang; Weili Zhou; Suqing Wu; Shengbing He; Jungchen Huang; Linkui Cao

doi:10.1016/j.scitotenv.2018.06.185

The influence of phosphorus on the autotrophic and mixotrophic denitrification

Sci Total Environ. 2018 Dec 1:643:127-133. doi: 10.1016/j.scitotenv.2018.06.185. Epub 2018 Jun 21.

Authors

Chunzhen Fan¹, Peiqi Wang², Weili Zhou³, Suqing Wu⁴, Shengbing He⁴, Jungchen Huang⁴, Linkui Cao⁵

Affiliations

¹ School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China; School of Agriculture and Biology, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China.
² School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China; Shanghai Solid Waste Disposal Co., Ltd., No. 2491, Jiazhu Highway, Jiading District, Shanghai 201815, China.
³ School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China. Electronic address: weilizhou@sjtu.edu.cn.
⁴ School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China.
⁵ School of Agriculture and Biology, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China.

PMID: 29936156
DOI: 10.1016/j.scitotenv.2018.06.185

Abstract

Autotrophic and mixotrophic denitrification, two approaches of biological denitrification, have drawn more and more attention among the techniques to remove nitrogen from the aquatic environment. This study investigated the influence of phosphorus on the denitrification performance and bacterial community structure in the autotrophic and mixotrophic denitrification reactors. The activity test was applied to evaluate the variation of denitrification activity of autotrophic and mixotrophic sludge before and after phosphorus addition. High-throughput sequencing was used to analyze the change of bacterial community structure. The results showed that NO₃^--N removal efficiency of autotrophic and mixotrophic denitrification process increased by 40 and 35%, respectively, after phosphorus addition. The sludge denitrification activity of autotrophic and mixotrophic sludge was enhanced significantly. And phosphorus addition could greatly improve the proportion of denitrifying bacteria in both autotrophic (from 11.83 to 64.31%) and mixotrophic denitrifying sludge (from 13.59 to 45.12%). Overall, phosphorus addition could greatly improve the autotrophic and mixotrophic denitrification ability in the phosphorus deficient surface water.

Keywords: Autotrophic and mixotrophic denitrification; Microbial community; Phosphorus; Sludge activity.

MeSH terms

Bioreactors
Denitrification*
Nitrates
Nitrogen
Phosphorus*
Sewage
Sulfates
Waste Disposal, Fluid / methods*

Substances

Nitrates
Sewage
Sulfates
Phosphorus
Nitrogen