Draft genome sequences of extended-spectrum β-lactamase-producing Enterobacter aerogenes isolated from swine and human

Luria Leslie Founou; Raspail Carrel Founou; Mushal Allam; Arshad Ismail; Sabiha Yusuf Essack

doi:10.1016/j.jgar.2018.06.006

Draft genome sequences of extended-spectrum β-lactamase-producing Enterobacter aerogenes isolated from swine and human

J Glob Antimicrob Resist. 2018 Sep:14:70-71. doi: 10.1016/j.jgar.2018.06.006. Epub 2018 Jun 20.

Authors

Luria Leslie Founou¹, Raspail Carrel Founou², Mushal Allam³, Arshad Ismail³, Sabiha Yusuf Essack⁴

Affiliations

¹ Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Food Safety and Environmental Microbiology, Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM), Yaoundé, Cameroon. Electronic address: luriafounou@gmail.com.
² Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Clinical Microbiology, Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM), Yaoundé, Cameroon.
³ Sequencing Core Facility, National Health Laboratory Service, Johannesburg, South Africa.
⁴ Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.

PMID: 29935332
DOI: 10.1016/j.jgar.2018.06.006

Abstract

Objectives: The draft genome sequences of two Enterobacter aerogenes strains (HN503E2II and PN108E5IIB) isolated from two Cameroonian abattoirs are reported.

Methods: Bacterial genomic DNA of the two isolates was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using CLC Genomics Workbench and SPAdes. The assembled contigs were annotated, and antibiotic resistance genes, virulence factors and plasmids were identified.

Results: Whole-genome sequencing revealed that both strains were similar, with genomes of 4878638bp and 4794257bp, encoding several resistance genes associated with resistance to β-lactams, fluoroquinolones, aminoglycosides, fosfomycin, phenicols, sulphonamides, trimethoprim, macrolides and tetracycline. In silico analysis also revealed chromosomal integration of one plasmid in the genome of PN108E5IIB.

Conclusion: The genome sequences reported here will provide useful information for a better understanding of the genetic structure of E. aerogenes in Africa.

Keywords: Antibiotic resistance; ESBL; Enterobacter aerogenes; Extended-spectrum β-lactamase; Food chain; Whole-genome sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abattoirs
Animals
Anti-Bacterial Agents / pharmacology
Cameroon
DNA, Bacterial / genetics
Drug Resistance, Multiple, Bacterial / genetics*
Enterobacter aerogenes / drug effects*
Enterobacter aerogenes / genetics*
Enterobacter aerogenes / isolation & purification
Enterobacteriaceae Infections / microbiology
Female
Genome, Bacterial*
Humans
Microbial Sensitivity Tests
Nose / microbiology
Plasmids / genetics
Swine
Virulence Factors / genetics
Whole Genome Sequencing
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
DNA, Bacterial
Virulence Factors
beta-Lactamases