Background: The use of saline in preserving bone specimens may affect the mechanical properties of specimens. Yet, the reported effects varied and contradicted to each other, with a lack of investigating constitutive material parameters. Therefore, we quantified the effects of preservation time on the constitutive properties of cortical bone.
Methods: We collected 120 specimens from the mid-diaphysis of six male bovine femora, which were assigned to five groups, including fresh-frozen for 60 days (-20 °C), storage in saline for 3, 10, 36 and 60 days (25 °C). All specimens underwent quasi-static three-point bending tests with a loading rate of 0.02 mm/s. Using the optimization method combined with specimen-specific finite element models, the Young's modulus, tangent modulus, yield stress, effective plastic strain, yield strain, ultimate stress, and toughness were calculated.
Findings: Saline preservation resulted in a significant decrease of Young's modulus, yield stress, ultimate stress and pre-yield toughness (P < 0.001), and a significant increase of effective plastic strain (P = 0.034). After 10 days of preservation, yield stress and pre-yield toughness decreased -14.9% and -21.4%, respectively, and they continued to decrease with longer preservation time. After 36 days of preservation, Young's modulus and ultimate stress decreased -19.2% and -17.3%, respectively, and continued to decrease with longer preservation time. Our data also showed changes of material properties collected after 3-day saline preservation, while the low statistical power must be considered for this group.
Interpretation: Saline preservation impacts on mechanical properties of cortical bone tissue and the effect is already observable after 3 days.
Keywords: Cortical bone; Finite element optimization; Material property; Saline preservation; Three-point bending.
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