Abstract
It was recently shown that removal of GreA, a transcription fidelity factor, enhances DNA break repair. This counterintuitive result, arising from unresolved backtracked RNA polymerase impeding DNA resection and thereby facilitating RecA-loading, leads to an interesting corollary: error-free full-length transcripts and broken chromosomes. Therefore, transcription fidelity may compromise genomic integrity.
Keywords:
Backtracked RNA polymerase; DNA break repair; DNA resection; DksA; Epimutation; Genetic noise; Genomic integrity; GreAB; RecBCD; Transcription fidelity.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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DNA / genetics
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DNA / metabolism
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DNA Repair
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DNA Replication*
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DNA-Directed RNA Polymerases / genetics
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DNA-Directed RNA Polymerases / metabolism
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Epigenesis, Genetic
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Escherichia coli / genetics*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Genome, Bacterial*
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Single-Cell Analysis
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcription, Genetic*
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Transcriptional Elongation Factors / genetics
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Transcriptional Elongation Factors / metabolism
Substances
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Escherichia coli Proteins
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GreA protein, E coli
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GreB protein, E coli
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Transcription Factors
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Transcriptional Elongation Factors
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dksA protein, E coli
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DNA
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DNA-Directed RNA Polymerases