2'-O,4'-C-Ethylene-bridged nucleic acid (ENA) is a sugar-modified oligonucleotide with an ethylene bridge between the 2'-oxygen and 4'-carbon of ribose. ENA not only has as high binding affinity to complementary RNA as conventional bridged/locked nucleic acid, but also has much higher nuclease resistance in plasma, which makes it a promising candidate for antisense therapeutics. This unit presents detailed protocols for the synthesis and characterization of ENA nucleosides and oligonucleotides. © 2018 by John Wiley & Sons, Inc.
Keywords: 2′-O,4′-C-ethylene-bridged nucleic acid (ENA); antisense; bridged nucleic acid (BNA); locked nucleic acid (LNA); sugar-modified nucleoside.
© 2018 John Wiley & Sons, Inc.