[High sensitive ELISA for detection of atrazion]

Xiao-Li Li; Ting-Ting Wang; Kai Liu; Bao-An Ning; Xin-Hua Ma; Nan Liu; Guo-Rong Ou; Zhi-Xian Gao; Zhong-Wen Liu

doi:10.12047/j.cjap.5582.2018.045

[High sensitive ELISA for detection of atrazion]

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 Feb 8;34(2):187-192. doi: 10.12047/j.cjap.5582.2018.045.

[Article in Chinese]

Authors

Xiao-Li Li¹, Ting-Ting Wang¹, Kai Liu¹, Bao-An Ning¹, Xin-Hua Ma¹, Nan Liu¹, Guo-Rong Ou¹, Zhi-Xian Gao¹, Zhong-Wen Liu¹

Affiliation

¹ Institute of Environmental and Operational Medicine, Military Medical Research Institute of Military Academy Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin 300050, China.

PMID: 29926688
DOI: 10.12047/j.cjap.5582.2018.045

Abstract

Objectives: To set up ELISA for detection of atrazine with high precision.

Methods: The reaction condition of indirect-ELISA was optimized, including atrazine-ovalbumin(AT-OVA) concentration and primary antibody concentration, organic solvent, goat anti-rat immunoglobin G-horseradish peroxidase(IgG-HRP) concentration. The actual samples were detected by the ELISA method established in our laboratory. Then the ELISA method was compared with the HPLC.

Results: The specification curve of indirect-ELISA was set up after optimization. The relation coefficient R²=0.9958. The limit of detection (LOD) was 1.972 ng/ml. The percent recovery of the actual samples was in range of 80%~120%. The ELISA detection sensitivity was higher than the HPLC in the range of 0 ng/ml~6 ng/ml atrazine.

Conclusions: The ELISA to detect atrazine has good specificity and high precision. And it can be applied in testing real atrazine samples replacing of the large-scale instrument.

Keywords: ELISA; atrazine; detection.

MeSH terms

Animals
Atrazine / analysis*
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay*
Sensitivity and Specificity

Substances

Atrazine