Synthesis of DNA-Based Nanowires

Alexander Kotlyar

doi:10.1007/978-1-4939-8582-1_3

Synthesis of DNA-Based Nanowires

Methods Mol Biol. 2018:1811:23-47. doi: 10.1007/978-1-4939-8582-1_3.

Author

Alexander Kotlyar¹

Affiliation

¹ Department of Biochemistry & Molecular Biology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel. s2shak@post.tau.ac.il.

PMID: 29926444
DOI: 10.1007/978-1-4939-8582-1_3

Abstract

Here we describe novel enzymatic procedures for the production of long (from tens of nanometers to microns) double-stranded poly(dG)-poly(dC), triple-helical poly(dG)-poly(dG)-poly(dC), and quadruple-helical G4 DNA. All these molecules are uniform in size and possess improved mechanical and electrical properties with respect to a canonical random sequence double-stranded DNA. They can potentially be used as elements in nanoelectronic devices and circuits.

Keywords: DNA nanowires; Enzymatic synthesis; G4-DNA; Klenow exo−; Poly(dG)–poly(dC); Triplex DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemical synthesis
DNA / chemistry
DNA Polymerase I / metabolism
Nanowires / chemistry*
Polydeoxyribonucleotides / chemical synthesis*
Polydeoxyribonucleotides / chemistry

Substances

Polydeoxyribonucleotides
poly(dG).poly(dC)
DNA
DNA Polymerase I