Visualization of Brain Gangliosides Using MALDI Imaging Mass Spectrometry

Eiji Sugiyama; Mitsutoshi Setou

doi:10.1007/978-1-4939-8552-4_10

Visualization of Brain Gangliosides Using MALDI Imaging Mass Spectrometry

Methods Mol Biol. 2018:1804:223-229. doi: 10.1007/978-1-4939-8552-4_10.

Authors

Eiji Sugiyama¹, Mitsutoshi Setou²

Affiliations

¹ Department of Biochemistry, Keio University School of Medicine, Tokyo, Japan.
² International Mass Imaging Center and Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Shizuoka, Japan. setou@hama-med.ac.jp.

PMID: 29926411
DOI: 10.1007/978-1-4939-8552-4_10

Abstract

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a histological method used for various molecules including gangliosides. The method is based on mass spectrometry, and thus target molecules with small structural differences are directly and independently detected. Here we describe a general procedure and related key notes for analyzing major brain gangliosides by MALDI-IMS.

Keywords: Brain; Ganglioside; Glycosphingolipids; Imaging mass spectrometry; Matrix-assisted laser desorption/ionization.

MeSH terms

Animals
Brain / metabolism*
Cryoultramicrotomy
Data Analysis
Gangliosides / metabolism*
Mice, Inbred C57BL
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Gangliosides