In Vitro Study of Antitumor Effect of Antimicrobial Peptide Tachyplesin I in Combination with Cisplatin

D V Kuzmin; A A Emel'yanova; M B Kalashnikova; P V Panteleev; T V Ovchinnikova

doi:10.1007/s10517-018-4134-6

In Vitro Study of Antitumor Effect of Antimicrobial Peptide Tachyplesin I in Combination with Cisplatin

Bull Exp Biol Med. 2018 Jun;165(2):220-224. doi: 10.1007/s10517-018-4134-6. Epub 2018 Jun 19.

Authors

D V Kuzmin¹, A A Emel'yanova¹, M B Kalashnikova¹, P V Panteleev¹, T V Ovchinnikova²

Affiliations

¹ M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
² M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. ovch@ibch.ru.

PMID: 29922995
DOI: 10.1007/s10517-018-4134-6

Abstract

We studied combined effect of β-hairpin antimicrobial peptide tachyplesin I and cytotoxic agent cisplatin on tumor and normal human cell lines. MTT assay and flow cytometry showed that tachyplesin I selectively sensitized cancer cells to cisplatin in specified concentration ratios. In vitro experiments demonstrated that combined use of tachyplesin I and cisplatin allows decreasing the effective dose of the cytostatic thus reducing nonspecific toxicity.

Keywords: antimicrobial peptide; cisplatin; cytotoxicity; effective dose; tachyplesin.

MeSH terms

Anti-Infective Agents / administration & dosage
Anti-Infective Agents / pharmacology
Antimicrobial Cationic Peptides / administration & dosage
Antimicrobial Cationic Peptides / pharmacology*
Antineoplastic Combined Chemotherapy Protocols / pharmacology*
Cell Proliferation / drug effects
Cells, Cultured
Cisplatin / administration & dosage
Cisplatin / pharmacology*
DNA-Binding Proteins / administration & dosage
DNA-Binding Proteins / pharmacology*
Dose-Response Relationship, Drug
Drug Interactions
Drug Screening Assays, Antitumor
HEK293 Cells
HL-60 Cells
Humans
Neoplasms / pathology*
Peptides, Cyclic / administration & dosage
Peptides, Cyclic / pharmacology*

Substances

Anti-Infective Agents
Antimicrobial Cationic Peptides
DNA-Binding Proteins
Peptides, Cyclic
tachyplesin peptide, Tachypleus tridentatus
Cisplatin