Development of Mobile Laboratory for Viral Hemorrhagic Fever Detection in Africa

J Infect Dis. 2018 Oct 5;218(10):1622-1630. doi: 10.1093/infdis/jiy362.

Abstract

Background: A mobile laboratory transportable on commercial flights was developed to enable local response to viral hemorrhagic fever outbreaks.

Methods: The development progressed from use of mobile real-time reverse-transcription polymerase chain reaction to mobile real-time recombinase polymerase amplification. In this study, we describe various stages of the mobile laboratory development.

Results: A brief overview of mobile laboratory deployments, which culminated in the first on-site detection of Ebola virus disease (EVD) in March 2014, and their successful use in a campaign to roll back EVD cases in Conakry in the West Africa Ebola virus outbreak are described.

Conclusions: The developed mobile laboratory successfully enabled local teams to perform rapid disgnostic testing for viral hemorrhagic fever.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Africa, Western
  • Ebolavirus / genetics
  • Hemorrhagic Fever, Ebola / diagnosis*
  • Hemorrhagic Fever, Ebola / virology
  • Humans
  • Laboratories*
  • Mobile Health Units*
  • Molecular Typing / instrumentation
  • Point-of-Care Systems*
  • RNA, Viral / blood
  • Real-Time Polymerase Chain Reaction / instrumentation

Substances

  • RNA, Viral