Different forms of copper and kinetin impacted element accumulation and macromolecule contents in kidney bean (Phaseolus vulgaris) seeds

Suzanne A Apodaca; Illya A Medina-Velo; Alek C Lazarski; Juan P Flores-Margez; Jose R Peralta-Videa; Jorge L Gardea-Torresdey

doi:10.1016/j.scitotenv.2018.04.360

Different forms of copper and kinetin impacted element accumulation and macromolecule contents in kidney bean (Phaseolus vulgaris) seeds

Sci Total Environ. 2018 Sep 15:636:1534-1540. doi: 10.1016/j.scitotenv.2018.04.360. Epub 2018 May 9.

Authors

Suzanne A Apodaca¹, Illya A Medina-Velo², Alek C Lazarski³, Juan P Flores-Margez⁴, Jose R Peralta-Videa⁵, Jorge L Gardea-Torresdey⁶

Affiliations

¹ Environmental Science and Engineering Ph.D. Program, The University of Texas at El Paso, 500 W. University Avenue, El Paso 79968, TX, United States; University of California Center for Environmental Implications of Nanotechnology (UC CEIN), The University of Texas at El Paso, 500 West University Ave., El Paso 79968, TX, United States.
² Department of Chemistry and Biochemistry, The University of Texas at El Paso, 500 W. University Avenue, El Paso 79968, TX, United States; University of California Center for Environmental Implications of Nanotechnology (UC CEIN), The University of Texas at El Paso, 500 West University Ave., El Paso 79968, TX, United States.
³ Department of Chemistry and Biochemistry, The University of Texas at El Paso, 500 W. University Avenue, El Paso 79968, TX, United States.
⁴ Autonomous University of Ciudad Juárez, Departamento de Química y Biología, Instituto de Ciencias Biomédicas, Anillo envolvente PRONAF y Estocolmo, Ciudad Juárez, Chihuahua 32310, Mexico.
⁵ Environmental Science and Engineering Ph.D. Program, The University of Texas at El Paso, 500 W. University Avenue, El Paso 79968, TX, United States; Department of Chemistry and Biochemistry, The University of Texas at El Paso, 500 W. University Avenue, El Paso 79968, TX, United States; University of California Center for Environmental Implications of Nanotechnology (UC CEIN), The University of Texas at El Paso, 500 West University Ave., El Paso 79968, TX, United States.
⁶ Environmental Science and Engineering Ph.D. Program, The University of Texas at El Paso, 500 W. University Avenue, El Paso 79968, TX, United States; Department of Chemistry and Biochemistry, The University of Texas at El Paso, 500 W. University Avenue, El Paso 79968, TX, United States; University of California Center for Environmental Implications of Nanotechnology (UC CEIN), The University of Texas at El Paso, 500 West University Ave., El Paso 79968, TX, United States. Electronic address: jgardea@utep.edu.

PMID: 29913614
DOI: 10.1016/j.scitotenv.2018.04.360

Abstract

The relationship between engineered nanomaterials and plant biostimulants is unclear. In this study, kidney bean (Phaseolus vulgaris) plants were grown to maturity (90 days) in soil amended with nano copper (nCu), bulk copper (bCu), or copper chloride (CuCl₂) at 0, 50, or 100 mg kg^-1, then watered with 0, 10, or 100 μM of kinetin (KN). Seeds were harvested and analyzed via ICP-OES and biochemical assays. While seed production was largely unaffected, nutritional value was significantly impacted. Accumulation of Cu was enhanced by 5-10% from controls by Cu-based treatments. Fe was the only macro/microelement significantly altered by nCu, which was ~29% lower than seeds from untreated plants. All forms of Cu combined with 10 μM KN reduced Mg from 9 to 12%. Application of KN plus bCu or CuCl₂ elevated concentrations of Mn (31-41%) and S (19-22%), respectively. Protein content of seeds was stimulated (11-12%) by bCu, on average, and depressed by CuCl₂ + KN (up to 22%). Variations in sugar and starch content were insignificant, compared to controls. Our results indicate that the interaction Cu × KN significantly altered the nutritional value of common beans, which has potential implications to agricultural practices incorporating Cu as either a pesticide or fertilizer.

Keywords: Copper; Kidney beans; Kinetin; Nanoparticles; Nutritional quality; Phytohormones.

MeSH terms

Copper / analysis*
Fertilizers
Kinetin / analysis*
Nutritive Value
Phaseolus / chemistry*
Seeds / chemistry
Soil
Soil Pollutants / analysis*

Substances

Fertilizers
Soil
Soil Pollutants
Copper
Kinetin
cupric chloride