DNA sequence features in the establishing of H3K27ac

Anatoliy Zubritskiy; Yulia A Medvedeva

doi:10.12688/f1000research.13441.2

DNA sequence features in the establishing of H3K27ac

F1000Res. 2018 Feb 8:7:165. doi: 10.12688/f1000research.13441.2. eCollection 2018.

Authors

Anatoliy Zubritskiy¹, Yulia A Medvedeva^{1

2

3}

Affiliations

¹ Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russian Federation.
² Department of Computational Biology, Vavilov institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russian Federation.
³ Department of Biological and Medical Physics, Moscow Institute of Physics and Technology, Dolgoprudny, 141701, Russian Federation.

Abstract

The presence of H3K27me3 has been demonstrated to correlate with the CpG content. In this work, we tested whether H3K27ac has similar sequence preferences. We performed a translocation of DNA sequences with various properties into a beta-globin locus to control for the local chromatin environment. Our results suggest that in contrast to H3K27me3, H3K27ac gain is unlikely affected by the CpG content of the underlying DNA sequence, while extremely high GC-content might contribute to the gain of the H3K27ac.

Keywords: CpG dinucleotides; GC-content; H3K27ac; histone modification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylation
Chromatin / chemistry*
Chromatin / genetics
CpG Islands / genetics*
DNA Methylation*
Histones / chemistry*
Histones / genetics
Humans
Protein Processing, Post-Translational*
Sequence Analysis, DNA / methods*
beta-Globins / genetics*

Substances

Chromatin
Histones
beta-Globins

Grants and funding

This work was supported by the Russian Science Foundation [15-14-30002].