Objective: To investigate the protective effect of proanthocyanidin on oxidative stress and mRNA expression of Nrf2 and HO-1 of mice cerebellar tissue induced by cypermethrin.
Methods: A total of 50 male Kunming mice were randomly divided into five groups by gavage once daily for three consecutive weeks, the control group, CYP group( 10 mg / kg) and PC pretreated group( 50, 100 and 200 mg / kg). The mice were sacrificed and activitie levels of GSH-Px, T-SOD, CAT and MDA were measured, and then used the immunohistochemistry to analyse the expression of Nrf2, semi-quantitative RTPCR detected mRNA expression of Nrf2 and HO-1.
Results: Compared to the controlgroup, the activity levels of GSH-Px, T-SOD, CAT decreased and MDA increased in CYP group. Nucleus positive cells was higher and mRNA expression of Nrf2 and HO-1 was higher than the control group( P < 0. 05). Compared to the CYP group, the level of GSHPx、T-SOD、CAT increased and MDA decreased in PC pretreated group, the count of nucleus-positive cells and mRNA expression of Nrf2 and HO-1 was lower than the CYP group( P < 0. 05).
Conclusion: s Cypermethrin could increase oxidative stress in cerebellar tissue of mice, induces the nuclear translocation of Nrf2 from the cytoplasm to the nucleus, and upregulating downstream gene of HO-1 expression. Proanthocyanidin could adjust mRNA expression of Nrf2 and HO-1 and ameliorate cypermethrin-induced oxidative stress.
Keywords: cypermethrin; heme oxygenase-1; nuclear factor-erythroid 2-related factor 2; proanthocyanidin.