Objective: A method for the determination of 6-benzylaminopurine( 6-BAP), isopentennyladenine( z-IP), 4-fluorophenoxyacetic acid( 4-FPA), 4-chlorophenoxyacetic acid( 4-CPA) in bean sprout was developed using solid phase extraction column with ultra-high performance liquid chromatography.
Methods: The sample was extracted by acetonitrile,dehydrated by salt,then centrifugation,and purified by PXC/PWA solid phase extraction column. The chromatographic analysis was carried out on C18 chromatographic column( 100 mm ×2. 1 mm,1. 8 μm),acetonitrile and sodium dihydrogen phosphate for gradient elution,diode array detector for detection,and quantified with external standard method.
Results: The calibration curves showed good linearity in the range of 0. 25-25 μg/mL( 6-BAP and z-IP) and 0. 50-50 μg/mL( 4-FPA and 4-CPA) with correlation coefficients greater than 0. 999. Three levels spiked recoveries were carried out using blank bean sprout extraction as substrate,the recoveries ranged from70. 0% to 96. 4%,and the relative standard deviations( RSDs) ranged from 2. 84% to12. 10%( n = 6). The qualitative limits of detections were 0. 0082-0. 075 mg/kg and the quantitative limits were 0. 027-0. 25 mg/kg for the 4 PGRs.
Conclusion: The method is simple and easy to operate using solid phase extraction column coupled,simultaneous determination of 4 PGRs by ultra-high performance liquid chromatography,can ensure the corresponding accuracy,sensitivity and precision.
Keywords: bean sprout; plant growth regulator; solid phase extraction column; ultra-high performance liquid chromatography(UPLC).