[Carboxylated hapten coated directly coated ELISA for the detection of atrazine residue in water]

Wei Sheng Yan Jiu. 2017 Jan;46(1):109-119.
[Article in Chinese]

Abstract

Objective: To develop the carboxylated hapten coated enzyme-linked immunosorbent assay( ELISA) for the detection of atrazine in drinking water.

Methods: Polystyrene surface was modified by 3-aminopropyltriethoxysilane( APTES) to produce amino groups for the directly immobilization of carboxylated atrazine on the surface of microtiter plates.

Results: The carboxylated hapten coated directly coated ELISA showed higher sensitivity( 0. 68 ng / m L) and higher specificity. In real sample analysis, the recoveries were ranged from 94. 0% to 112. 0%, and the relative standard deviation was 2. 72%- 3. 53%.

Conclusion: The method is simple, reliable, and can be used to detect atrazine in drinking water.

Keywords: ELISA; atrazine; carboxylated hapten; direct coated format.

MeSH terms

  • Atrazine / analysis*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Haptens / immunology*
  • Herbicides / analysis*
  • Herbicides / immunology
  • Polystyrenes
  • Propylamines
  • Sensitivity and Specificity
  • Silanes
  • Water / analysis*

Substances

  • Haptens
  • Herbicides
  • Polystyrenes
  • Propylamines
  • Silanes
  • Water
  • amino-propyl-triethoxysilane
  • Atrazine