Objective: To develop the carboxylated hapten coated enzyme-linked immunosorbent assay( ELISA) for the detection of atrazine in drinking water.
Methods: Polystyrene surface was modified by 3-aminopropyltriethoxysilane( APTES) to produce amino groups for the directly immobilization of carboxylated atrazine on the surface of microtiter plates.
Results: The carboxylated hapten coated directly coated ELISA showed higher sensitivity( 0. 68 ng / m L) and higher specificity. In real sample analysis, the recoveries were ranged from 94. 0% to 112. 0%, and the relative standard deviation was 2. 72%- 3. 53%.
Conclusion: The method is simple, reliable, and can be used to detect atrazine in drinking water.
Keywords: ELISA; atrazine; carboxylated hapten; direct coated format.