Abstract
A polymerase ribozyme can be used to label the 3' end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs. Guided by a complementary template, the ribozyme adds a single nucleotide that may contain a fluorophore, biotin, azide or alkyne moiety, thus enabling the detection and/or capture of selectively labeled materials. Employing a variety of commercially available nucleotide analogs, efficient labeling was demonstrated for model RNAs and DNAs, human microRNAs and natural tRNA.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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3' Flanking Region*
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Biotin / chemistry
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Biotin / metabolism
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DNA / chemistry
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DNA / metabolism
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DNA-Directed RNA Polymerases / metabolism*
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Fluorescein / chemistry
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Fluorescein / metabolism
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism
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MicroRNAs / chemistry
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MicroRNAs / metabolism
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Nucleic Acid Conformation
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Nucleic Acids / chemistry
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Nucleic Acids / metabolism*
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Nucleotidyltransferases / metabolism
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RNA / chemistry
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RNA / metabolism
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RNA, Catalytic / metabolism*
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RNA, Transfer / chemistry
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RNA, Transfer / metabolism
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Rhodamines / chemistry
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Rhodamines / metabolism
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Staining and Labeling / methods*
Substances
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Fluorescent Dyes
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MicroRNAs
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Nucleic Acids
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RNA, Catalytic
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Rhodamines
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RNA
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Biotin
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DNA
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RNA, Transfer
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Nucleotidyltransferases
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DNA-Directed RNA Polymerases
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Fluorescein