A dual functional fluorogenic probe for visualization of intracellular pH and formaldehyde with distinct fluorescence signals

Zhenda Xie; Biao Yin; Jiajie Shen; Danqi Hong; Liquan Zhu; Jingyan Ge; Qing Zhu

doi:10.1039/c8ob00989a

A dual functional fluorogenic probe for visualization of intracellular pH and formaldehyde with distinct fluorescence signals

Org Biomol Chem. 2018 Jul 7;16(25):4628-4632. doi: 10.1039/c8ob00989a. Epub 2018 Jun 14.

Authors

Zhenda Xie¹, Biao Yin¹, Jiajie Shen², Danqi Hong¹, Liquan Zhu¹, Jingyan Ge¹, Qing Zhu¹

Affiliations

¹ Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, ChaowangRoad 18, Hangzhou 310014, China. zhuq@zjut.edu.cn.
² Key Laboratory of Women's Reproductive Health of Zhejiang Province, Women's Hospital School of Medicine Zhejiang University, Xueshi Road 1, Hangzhou 310006, China.

PMID: 29901667
DOI: 10.1039/c8ob00989a

Abstract

In this study, a single fluorescent probe (DPFP) containing a 1,8-naphthalimide dye and a homoallylamino group for imaging pH and formaldehyde (FA) has been developed that exhibits significant blue fluorescence (λ_em at 455 nm) under acidic pH conditions (pH < 7.0) and green fluorescence (λ_em at 555 nm) in the presence of FA, respectively. Furthermore, probe DPFP was successfully applied to image acidic lysosomes and exogenous or endogenous FA in living HeLa cells.