Data on volatile compounds produced by serotype D Clostridium botulinum

Satoshi Nojima; Takao Myoda; Kazuki Toeda; Koichi Niwa; Toshihiro Watanabe; Yoshimasa Sagane

doi:10.1016/j.dib.2018.05.057

Data on volatile compounds produced by serotype D Clostridium botulinum

Data Brief. 2018 May 23:19:393-397. doi: 10.1016/j.dib.2018.05.057. eCollection 2018 Aug.

Authors

Satoshi Nojima¹, Takao Myoda¹, Kazuki Toeda¹, Koichi Niwa¹, Toshihiro Watanabe¹, Yoshimasa Sagane¹

Affiliation

¹ Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri Hokkaido 099-2493, Japan.

Abstract

We analyzed the volatile compounds produced by serotype D Clostridium botulinum (D-CB16) in trypticase peptone/yeast extract/glucose (TYG) medium using gas chromatography/mass spectrometry (GC/MS). The volatile compounds were captured by solid-phase microextraction and applied to GC/MS for separation and identification of the compounds in TYG medium with or without the cultivation of C. botulinum D-CB16. Thirty-five and 34 volatile compounds were identified in media without and with D-CB16 cultivation, respectively. Of the compounds identified in the medium with the strain, twenty-one were not detected in the original medium, indicating that these were produced by C. botulinum D-CB16.

Keywords: Botulism; Clostridium botulinum; DVB/CAR/PDMS, divinylbenzene/carboxen/polydimethylsiloxane; EI, Electron impact; Food poisoning; GC, gas chromatography; Gas chromatography/mass spectrometry; MS, mass spectrometry; SPME, Solid-phase microextraction; TYG, trypticase peptone/yeast extract/glucose; Volatile compounds.