Dataset of cathepsin L-like CP inhibition of Naegleria fowleri and Acanthamoeba castellanii by ppTvCP4r from Trichomonas vaginalis

Rosa E Cárdenas-Guerra; Moisés Martínez-Castillo; Jaime Ortega-López; Mineko Shibayama; Rossana Arroyo

doi:10.1016/j.dib.2018.03.029

Dataset of cathepsin L-like CP inhibition of Naegleria fowleri and Acanthamoeba castellanii by ppTvCP4r from Trichomonas vaginalis

Data Brief. 2018 Mar 13:18:404-408. doi: 10.1016/j.dib.2018.03.029. eCollection 2018 Jun.

Authors

Rosa E Cárdenas-Guerra¹, Moisés Martínez-Castillo¹, Jaime Ortega-López², Mineko Shibayama¹, Rossana Arroyo¹

Affiliations

¹ Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN # 2508, Col. San Pedro Zacatenco, Delg. Gustavo A. Madero, CP 07360 Ciudad de México, Mexico.
² Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN # 2508, Col. San Pedro Zacatenco, Delg. Gustavo A. Madero, CP 07360 Ciudad de México, Mexico.

Abstract

The recombinant TvCP4 prepro region (ppTvCP4r) acts as an exogenous inhibitor of cathepsin L-like CPs from Trichomonas vaginalis (Cárdenas-Guerra et al., 2015 [1]). Here, we present the dataset of the trichomonad ppTvCP4r inhibitory effect against the CP proteolytic activities from other microorganisms, such as Naegleria fowleri and Acanthamoeba castellanii free-living amoeba. The proteolytic activity inhibition of total crude extracts (TCEs) of N. fowleri and A. castellanii was determined and recorded using a fluorogenic substrate specific for cathepsin L CPs without or with a ppTvCP4r treatment at different concentrations and pH.

Keywords: Acanthamoeba castellanii; Cathepsin L-like CPs; Cysteine proteinase inhibitors; Naegleria fowleri; Trichomonas vaginalis; ppTvCP4r.