Benzodiazepine receptor binding in vivo with [3H]-Ro 15-1788

Life Sci. 1985 Jul 29;37(4):345-55. doi: 10.1016/0024-3205(85)90505-3.

Abstract

In vivo benzodiazepine receptor binding has generally been studied by "ex vivo" techniques. In this investigation, we identify the conditions where [3H]-Ro 15-1788 labels benzodiazepine receptors by true "in vivo" binding, i.e. where workable specific to nonspecific ratios are obtained in intact tissues without homogenization or washing. [3H]-Flunitrazepam and [3H]-clonazepam did not exhibit useful in vivo receptor binding.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Benzodiazepinones / metabolism*
  • Brain / metabolism
  • Clonazepam / metabolism
  • Flumazenil
  • Flunitrazepam / metabolism
  • In Vitro Techniques
  • Male
  • Mice
  • Mice, Inbred ICR
  • Receptors, GABA-A / metabolism*
  • Time Factors
  • Tritium

Substances

  • Benzodiazepinones
  • Receptors, GABA-A
  • Tritium
  • Flumazenil
  • Clonazepam
  • Flunitrazepam