Vectors with restriction-site banks. III. Escherichia coli-Saccharomyces cerevisiae shuttle vectors

Gene. 1985;34(2-3):363-6. doi: 10.1016/0378-1119(85)90146-5.

Abstract

The bank of unique restriction sites present in plasmid pJRD158 has been incorporated into new vectors carrying selective markers and replicons derived from commonly used Escherichia coli-Saccharomyces cerevisiae shuttle vectors pJDB207 and YRp7. The new vectors pMH158 and pJO158 have 21 and 23 unique restriction sites, respectively, and their complete DNA sequences are known.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • Escherichia coli / genetics*
  • Genetic Vectors*
  • Plasmids
  • Saccharomyces cerevisiae / genetics*

Substances

  • DNA, Recombinant
  • DNA Restriction Enzymes