Generation and Characterization of Stable α-Synuclein Oligomers

Leire Almandoz-Gil; Martin Ingelsson; Joakim Bergström

doi:10.1007/978-1-4939-7816-8_5

Generation and Characterization of Stable α-Synuclein Oligomers

Methods Mol Biol. 2018:1779:61-71. doi: 10.1007/978-1-4939-7816-8_5.

Authors

Leire Almandoz-Gil¹, Martin Ingelsson¹, Joakim Bergström²

Affiliations

¹ Molecular Geriatrics, Department of Public Health and Caring Sciences, Uppsala University, Uppsala, Sweden.
² Molecular Geriatrics, Department of Public Health and Caring Sciences, Uppsala University, Uppsala, Sweden. joakim.bergstrom@pubcare.uu.se.

PMID: 29886527
DOI: 10.1007/978-1-4939-7816-8_5

Abstract

Alpha-synuclein oligomers are linked to the pathogenesis of Parkinson's disease and related neurodegenerative diseases. In this chapter, we present a method to generate kinetically stable α-synuclein oligomers by the addition of reactive aldehydes, 4-hydroxy-2-nonenal, and 4-oxo-2-nonenal. We also describe biochemical and immunological techniques to characterize the generated oligomers.

Keywords: 4-Hydroxy-2-nonenal; 4-Oxo-2-nonenal; Aldehydes; Oligomers; Oxidative stress; α-Synuclein.

MeSH terms

Aldehydes / chemistry*
Electrophoresis, Polyacrylamide Gel
Humans
Microscopy, Atomic Force
Parkinson Disease / metabolism*
Protein Multimerization
Protein Stability
alpha-Synuclein / chemical synthesis*
alpha-Synuclein / chemistry

Substances

4-oxo-2-nonenal
Aldehydes
alpha-Synuclein
4-hydroxy-2-nonenal