Structure and gene cluster of the K125 capsular polysaccharide from Acinetobacter baumannii MAR13-1452

Nikolay P Arbatsky; Mikhail M Shneider; Andrei S Dmitrenok; Anastasia V Popova; Dmitry A Shagin; Andrey A Shelenkov; Yulia V Mikhailova; Mikhail V Edelstein; Yuriy A Knirel

doi:10.1016/j.ijbiomac.2018.06.029

Structure and gene cluster of the K125 capsular polysaccharide from Acinetobacter baumannii MAR13-1452

Int J Biol Macromol. 2018 Oct 1:117:1195-1199. doi: 10.1016/j.ijbiomac.2018.06.029. Epub 2018 Jun 7.

Authors

Nikolay P Arbatsky¹, Mikhail M Shneider², Andrei S Dmitrenok¹, Anastasia V Popova³, Dmitry A Shagin⁴, Andrey A Shelenkov⁴, Yulia V Mikhailova⁴, Mikhail V Edelstein⁵, Yuriy A Knirel⁶

Affiliations

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
² M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia.
³ Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia; Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia; State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
⁴ Central Scientific Research Institute of Epidemiology, Moscow, Russia.
⁵ Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia.
⁶ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. Electronic address: yknirel@gmail.com.

PMID: 29886169
DOI: 10.1016/j.ijbiomac.2018.06.029

Abstract

A capsular polysaccharide (CPS) was isolated from strain MAR13-1452 of an emerging pathogen Acinetobacter baumannii and assigned type K125. The following structure of the CPS was established by sugar analysis, Smith degradation, and 1D and 2D ¹H and ¹³C NMR spectroscopy: Proteins encoded by the KL125 gene cluster in the genome of MAR13-1452, including three glycosyltransferases, were assigned roles in the biosynthesis of the K125 CPS.

Keywords: Acinetobacter baumannii; Bacterial polysaccharide structure; Capsular polysaccharide; Capsule biosynthesis; Gene cluster.

MeSH terms

Acinetobacter baumannii / chemistry*
Acinetobacter baumannii / classification
Acinetobacter baumannii / genetics*
Glycosyltransferases / chemistry
Glycosyltransferases / metabolism
Magnetic Resonance Spectroscopy
Multigene Family*
Polysaccharides, Bacterial / biosynthesis
Polysaccharides, Bacterial / chemistry*
Structure-Activity Relationship
Sugars / chemistry

Substances

Polysaccharides, Bacterial
Sugars
Glycosyltransferases