Generation of dendritic cells from both mouse and human tissues is a valuable technique for downstream immunotoxicological applications. Here, we describe methods for generation of four subsets of dendritic cells from murine bone marrow and three subsets of dendritic cells from human peripheral blood mononuclear cells.
Keywords: Bone marrow-derived dendritic cells (BMDCs); Cell culture; Dendritic cell; In vitro; Peripheral blood mononuclear cells (PBMCs).