Colorimetric detection of glucose using lanthanum-incorporated MCM-41

Abstract

In this study, lanthanum-containing mesoporous MCM-41 (La-MCM-41) with different amount of lanthanum were synthesized and were used for colorimetric detection of glucose. As prepared La-MCM-41 were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The quantitative amounts of incorporated lanthanum into MCM-41 structure were estimated by energy dispersive X-ray spectrometry. The prepared La-MCM-41 provided high intrinsic peroxidase-like activity in the presence of peroxidise 3,3',5,5'-tetramethylbenzidine (TMB) and H₂O₂ for accurate determination of glucose. This process produced a blue color in aqueous solution that directly relates to H₂O₂ concentration. The effect of different parameters such as the content of incorporated lanthanum, pH, temperature and time of reaction on the peroxidase-like activity was studied. The colorimetric detection of H₂O₂ was led to a linear dynamic range from 50 to 1000 μM (r² = 0.9988) and low detection limit of 37.5 μM for glucose in aqueous solution. These results are comparable (close to) or better than some previous reports. Thus, La-MCM-41 can be used as admirable alternative design for colorimetric sensing of glucose.

Keywords: Colorimetric detection; Enzyme mimetic compound; Glucose; La-MCM-41; Peroxidase.