Listeria monocytogenes is known as a major food-borne pathogen causing a severe life-threatening disease, listeriosis, in susceptible patients. This bacterium has special features that facilitate its survival in different conditions and cause resistance to antibacterial agents and biocides. Toxin-antitoxin (TA) system has a potential to be introduced as an antibacterial target because of its participation in cell physiology, including stress response, antiphage activity, biofilm formation, and resistance to antibiotics. In this study, after the identification of 6 genes of 3 TA pairs (lM/E-lM/F, lM/S-lM/B and ydc/D-ydc/E) via existing databases, the presence and expression level of these genes were investigated by PCR and q-PCR techniques, respectively. The result of RT-qPCR revealed that identified genes were expressed in different strains and ydc (maz) increased under thermal stress. It seems that the products of these genes play an important role in the physiology and survival of the bacterium especially in heat stress. Presence of 6 detected TA genes in all of the tested isolates demonstrated that TA system could be an antibacterial target in L. monocytogenes; however, more research is needed to explain the actual role of these genes.
Keywords: L. monocytogenes; PCR; Phylogenetic analysis; Toxin-antitoxin system; q-RT PCR.
Copyright © 2018. Published by Elsevier Ltd.