Across Eukaryota, DNA modifications play an important role in regulation of gene expression. While 5-methylcytosine (5mC) has been explored in depth, other modifications such as 6-methyladenine (6 mA) have historically been overlooked, in part due to technical difficulties in collecting/analyzing these data. However, recent technological advances have enabled exploration of these marks with much greater detail and on a larger scale. In this chapter, we discuss multiple methods for identifying and analyzing both 5mC and 6 mA across fungi.
Keywords: 5-Methylcytosine; 6 mA-immunoprecipitation followed by sequencing (6 mA-IP-seq); 6-Methyladenine; Bisulfite-sequencing; Epigenomics; Fungi; SMRT-analysis.