Fungal Transcriptomics

Vasanth R Singan; Rita C Kuo; Cindy Chen

doi:10.1007/978-1-4939-7804-5_8

Fungal Transcriptomics

Methods Mol Biol. 2018:1775:83-92. doi: 10.1007/978-1-4939-7804-5_8.

Authors

Vasanth R Singan¹, Rita C Kuo², Cindy Chen²

Affiliations

¹ United States Department of Energy Joint Genome Institute, Walnut Creek, CA, USA. VRSingan@lbl.gov.
² United States Department of Energy Joint Genome Institute, Walnut Creek, CA, USA.

PMID: 29876811
DOI: 10.1007/978-1-4939-7804-5_8

Abstract

Transcriptomics, the study of RNA molecules, provides in-depth understanding of cellular functions and the genomic landscape of transcription. Transcriptomics refers to the study of all classes on RNA molecules including mRNA, tRNA, and siRNA. In this chapter, we specifically focus on mRNA, which encodes the protein-coding portion of the genomic DNA. We discuss the use of mRNA in annotation of genomes and in studying differential regulation of genes under experimental conditions.

Keywords: Gene expression analysis; RNA-Seq; Transcriptome assembly; Transcriptomics.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Gene Expression Regulation, Fungal
Genome, Fungal / genetics*
Genomics / methods*
High-Throughput Nucleotide Sequencing / methods*
Molecular Sequence Annotation / methods
RNA, Messenger / genetics
Sequence Analysis, RNA / methods
Transcriptome / genetics*

Substances

RNA, Messenger