Spectroscopic analysis beyond the diffraction limit

Biqin Dong; Janel L Davis; Cheng Sun; Hao F Zhang

doi:10.1016/j.biocel.2018.06.002

Spectroscopic analysis beyond the diffraction limit

Int J Biochem Cell Biol. 2018 Aug:101:113-117. doi: 10.1016/j.biocel.2018.06.002. Epub 2018 Jun 3.

Authors

Biqin Dong¹, Janel L Davis², Cheng Sun³, Hao F Zhang⁴

Affiliations

¹ Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA; Department of Mechanical Engineering, Northwestern University, Evanston, IL, 60208, USA.
² Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA.
³ Department of Mechanical Engineering, Northwestern University, Evanston, IL, 60208, USA.
⁴ Department of Biomedical Engineering, Northwestern University, Evanston, IL, 60208, USA. Electronic address: hfzhang@northwestern.edu.

Abstract

The recent surge in spectroscopic Single-Molecule Localization Microscopy (sSMLM) offers exciting new capabilities for combining single molecule imaging and spectroscopic analysis. Through the synergistic integration of super-resolution optical microscopy and single-molecule spectroscopy, sSMLM offers combined strengths from both fields. By capturing the full spectra of single molecule fluorescent emissions, sSMLM can distinguish minute spectroscopic variations from individual fluorescent molecules while preserving nanoscopic spatial localization precision. It can significantly extend the coding space for multi-molecule super-resolution imaging. Furthermore, it has the potential to detect spectroscopic variations in fluorescence emission associated with molecular interactions, which further enables probing local chemical and biochemical inhomogeneities of the nano-environments. In this review, we seek to explain the working principle of sSMLM technologies and the status of sSMLM techniques towards new super-resolution imaging applications.

Keywords: Fluorescence spectral imaging; Single-molecule spectroscopy; Super-resolution microscopy.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Amyloid beta-Peptides / ultrastructure*
Animals
COS Cells
Chlorocebus aethiops
Fluorescent Dyes / chemistry
Humans
Hydrophobic and Hydrophilic Interactions
Lipid Bilayers / chemistry
Lipid Bilayers / metabolism
Microscopy, Fluorescence / instrumentation
Microscopy, Fluorescence / methods*
Molecular Imaging / instrumentation
Molecular Imaging / methods*
Oxazines / chemistry
Rhodamines / chemistry
Single Molecule Imaging / instrumentation
Single Molecule Imaging / methods*
alpha-Synuclein / ultrastructure*

Substances

Amyloid beta-Peptides
Fluorescent Dyes
Lipid Bilayers
Oxazines
Rhodamines
alpha-Synuclein
nile red

Abstract

Publication types

MeSH terms

Substances

Grants and funding