The Tomato Hybrid Proline-rich Protein regulates the abscission zone competence to respond to ethylene signals

Srivignesh Sundaresan; Sonia Philosoph-Hadas; Chao Ma; Cai-Zhong Jiang; Joseph Riov; Raja Mugasimangalam; Betina Kochanek; Shoshana Salim; Michael S Reid; Shimon Meir

doi:10.1038/s41438-018-0033-2

The Tomato Hybrid Proline-rich Protein regulates the abscission zone competence to respond to ethylene signals

Hortic Res. 2018 Jun 1:5:28. doi: 10.1038/s41438-018-0033-2. eCollection 2018.

Authors

Srivignesh Sundaresan^{1

2

3}, Sonia Philosoph-Hadas¹, Chao Ma^{4

5}, Cai-Zhong Jiang^{4

6}, Joseph Riov², Raja Mugasimangalam⁷, Betina Kochanek¹, Shoshana Salim¹, Michael S Reid⁴, Shimon Meir¹

Affiliations

¹ 1Department of Postharvest Science of Fresh Produce, Agricultural Research Organization (ARO), The Volcani Center, Rishon LeZiyon, Israel.
² 2The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
³ 6Present Address: Department of Nano Science and Technology, Tamil Nadu Agricultural University, Coimbatore, India.
⁴ 3Department of Plant Sciences, University of California, Davis, CA USA.
⁵ 7Present Address: Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University, Beijing, China.
⁶ 4Crops Pathology & Genetic Research Unit, USDA-ARS, Davis, CA USA.
⁷ Department of Bioinformatics, QTLomics Technologies Pvt. Ltd, Bangalore, India.

Abstract

The Tomato Hybrid Proline-rich Protein (THyPRP) gene was specifically expressed in the tomato (Solanum lycopersicum) flower abscission zone (FAZ), and its stable antisense silencing under the control of an abscission zone (AZ)-specific promoter, Tomato Abscission Polygalacturonase4, significantly inhibited tomato pedicel abscission following flower removal. For understanding the THyPRP role in regulating pedicel abscission, a transcriptomic analysis of the FAZ of THyPRP-silenced plants was performed, using a newly developed AZ-specific tomato microarray chip. Decreased expression of THyPRP in the silenced plants was already observed before abscission induction, resulting in FAZ-specific altered gene expression of transcription factors, epigenetic modifiers, post-translational regulators, and transporters. Our data demonstrate that the effect of THyPRP silencing on pedicel abscission was not mediated by its effect on auxin balance, but by decreased ethylene biosynthesis and response. Additionally, THyPRP silencing revealed new players, which were demonstrated for the first time to be involved in regulating pedicel abscission processes. These include: gibberellin perception, Ca²⁺-Calmodulin signaling, Serpins and Small Ubiquitin-related Modifier proteins involved in post-translational modifications, Synthaxin and SNARE-like proteins, which participate in exocytosis, a process necessary for cell separation. These changes, occurring in the silenced plants early after flower removal, inhibited and/or delayed the acquisition of the competence of the FAZ cells to respond to ethylene signaling. Our results suggest that THyPRP acts as a master regulator of flower abscission in tomato, predominantly by playing a role in the regulation of the FAZ cell competence to respond to ethylene signals.