[Effects of dihydroartemisinin on the apoptosis of Fadu cells]

L F Jia; X M Li; Y P Shen; X Y Lu

doi:10.13201/j.issn.1001-1781.2016.21.005

[Effects of dihydroartemisinin on the apoptosis of Fadu cells]

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2016 Nov 5;30(21):1685-1688. doi: 10.13201/j.issn.1001-1781.2016.21.005.

[Article in Chinese]

Authors

L F Jia¹, X M Li¹, Y P Shen¹, X Y Lu¹

Affiliation

¹ Department of Otolaryngology Head and Neck Surgery, Bethune International Peace Hospital, Shijiazhuang, 050082, China.

PMID: 29871174
DOI: 10.13201/j.issn.1001-1781.2016.21.005

Abstract

Objective:To investigate the effect of dihydroartemisinin (DHA) on the apoptosis of Fadu cells.Method:Fadu cells were treated with DMSO or different doses of DHA for 24 or 48 h.After that, IC50 values of DHA were calculated based on the cell viability tested via MTT assay, the cell morphology was observed,the percentage of apoptotic cells was analyzed with the Annexin V-FITC Apoptosis Detection Kit, and some important regulators of apoptosis, such as Bcl-2, Bcl-xl, Mcl-1, Bax, and C-PARP were determined by Western blotting.Result:Certain doses of DHA significantly inhibited the proliferation and induced apoptosis of Fadu cells in a dose and time dependent manners. DHA also downregulated the expression of antiapoptotic proteins (Bcl-2, Bcl-xl, and Mcl-1) and upregulated the expression of proapoptotic proteins (Bax and C-PARP).Conclusion:DHA remarkably inhibited proliferation and induction of apoptosis in Fadu cells via affecting proteins of Bcl-2 family. DHA may be a promising drug to treat hypopharyngeal carcinoma.

Keywords: Bcl-2; apoptosis; dihydroartemisinin; hypopharyngeal neoplasms.

Publication types

English Abstract