Dual Surface Modification of Genome-Free MS2 Capsids for Delivery Applications

Ioana L Aanei; Matthew B Francis

doi:10.1007/978-1-4939-7808-3_40

Dual Surface Modification of Genome-Free MS2 Capsids for Delivery Applications

Methods Mol Biol. 2018:1776:629-642. doi: 10.1007/978-1-4939-7808-3_40.

Authors

Ioana L Aanei^{1

2}, Matthew B Francis^{3

4}

Affiliations

¹ Department of Chemistry, University of California, Berkeley, CA, USA.
² Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, CA, USA.
³ Department of Chemistry, University of California, Berkeley, CA, USA. mbfrancis@berkeley.edu.
⁴ Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, CA, USA. mbfrancis@berkeley.edu.

PMID: 29869270
DOI: 10.1007/978-1-4939-7808-3_40

Abstract

One of the hallmarks of virus-like particles (VLPs) is the fact that they possess distinguishable interior and exterior surfaces. Taking advantage of our knowledge of the amino acid location from X-ray crystal structures, we have developed a series of synthetic modifications of the MS2 bacteriophage viral capsid, including small molecule and polymer attachment, as well as conjugation with peptides, DNA and other proteins. These constructs have found applications in nanomaterial fabrication and as delivery vehicles with therapeutic potential. Importantly, the dual-modification strategies described herein could be extended to other VLP systems.

Keywords: Antibodies; Aptamers; Bioconjugation; Delivery vehicle; Drugs; MS2 bacteriophage; Polymers; Site-specific modification; Virus-like particles.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Capsid Proteins / chemistry*
Levivirus / chemistry
Levivirus / genetics*
Peptides / chemistry
Peptides / genetics*
Virion / chemistry
Virion / genetics*

Substances

Capsid Proteins
Peptides

Grants and funding

R21 EB018055/EB/NIBIB NIH HHS/United States