Antioxidant status of rats' blood and liver affected by sodium selenite and selenium nanoparticles

Lenka Urbankova; Pavel Horky; Jiri Skladanka; Magdalena Pribilova; Vendula Smolikova; Pavel Nevrkla; Natalia Cernei; Zuzana Lackova; Josef Hedbavny; Andrea Ridoskova; Vojtech Adam; Pavel Kopel

doi:10.7717/peerj.4862

Antioxidant status of rats' blood and liver affected by sodium selenite and selenium nanoparticles

PeerJ. 2018 May 28:6:e4862. doi: 10.7717/peerj.4862. eCollection 2018.

Authors

Lenka Urbankova¹, Pavel Horky¹, Jiri Skladanka¹, Magdalena Pribilova¹, Vendula Smolikova^{2

3}, Pavel Nevrkla⁴, Natalia Cernei^{2

3}, Zuzana Lackova^{2

3}, Josef Hedbavny², Andrea Ridoskova^{2

3}, Vojtech Adam^{2

3}, Pavel Kopel^{2

3}

Affiliations

¹ Department of Animal Nutrition and Forage Production, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic.
² Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic.
³ Central European Institute of Technology, Technical University of Brno, Brno, Czech Republic.
⁴ Department of Animal Breeding, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic.

Abstract

Background: Selenium is an essential element; however, at higher doses, it can be toxic. Therefore, alternative nanotechnological solutions are required to overcome toxicological issues, rather than conventional alternatives. Nanoparticles show new and promising properties that may be able to suppress toxicity while maintaining the positive effects of selenium on an organism. The aim of the experiment was to determine the influence of sodium selenite and selenium nanoparticles (SeNPs) on the antioxidant status of rats.

Methods: The males of the outbreed rat strain Wistar albino were selected as a model organism. Animals were fed different forms of selenium. The control group was given a mixture without selenium addition, whereas other groups were fed a mixture containing sodium selenite, Se-49, and Se-100 SeNPs respectively. The duration of the trial was 30 days.

Results: Analysis of blood and liver was performed where the concentration of reduced (GSH) and oxidised (GSSG) glutathione, and total selenium content were measured. In the liver, a significant reduction in GSSG was found for all experiment groups. Blood samples showed a significant reduction in GSH and an increase in GSSG.

Discussion: These results show that SeNPs may be an alternative to dietary selenium for animal organisms.

Keywords: Animal nutrition; Antioxidant; Glutathione; Rat; Selenium nanoparticles.

Grants and funding

The study was supported by IP IGA MENDELU 17/2017. Moreover, this research was carried out under the project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.