Effects of Immunosenescence on the Lower Expression of Surface Molecules in Neutrophils and Lymphocytes

Alessandra B Lopes; Larissa B Lopes; Roseli N da Silveira Antunes; Josianne T Fukasawa; Débora de A Cavaretto; Zamir Calamita

doi:10.2174/1874609811666180605092234

Effects of Immunosenescence on the Lower Expression of Surface Molecules in Neutrophils and Lymphocytes

Curr Aging Sci. 2018;11(2):118-125. doi: 10.2174/1874609811666180605092234.

Authors

Alessandra B Lopes¹, Larissa B Lopes², Roseli N da Silveira Antunes³, Josianne T Fukasawa⁴, Débora de A Cavaretto⁴, Zamir Calamita⁵

Affiliations

¹ Postgraduate Program in Health and Aging, Marilia School of Medicine - FAMEMA, Rua Monte Carmelo, 800, Fragata, CEP: 17519-030, Marilia - SP, Brazil.
² Discipline of Hematology and Hemotherapy of the Federal University of Sao Paulo - UNIFESP, Rua Doutor Diogo de Faria, 824, Vila Clementino, CEP: 04037-002, Sao Paulo - SP, Brazil.
³ Discipline of Hematology and Hemotherapy of the School of Medicine of Marilia - FAMEMA. Rua Monte Carmelo, 800, Fragata, CEP: 17519-030, Marilia - SP, Brazil.
⁴ Laboratory of Flow Cytometry, Blood Center of Marilia Medical School - FAMEMA. Rua Lourival Freire, 240, Fragata, CEP: 17519-050, Marilia - SP, Brazil.
⁵ Discipline of Clinical Immunopathology and Allergy of the School of Medicine of Marilia - FAMEMA. Rua Monte Car-melo, 800, Fragata, CEP: 17519-030, Marilia - SP, Brazil.

PMID: 29866027
DOI: 10.2174/1874609811666180605092234

Abstract

Background: Immunosenescence is a remodeling of the immune system, caused by aging, with changes in the function of neutrophils, lymphocytes, and Treg cells.

Objective: This study aimed to evaluate the expression of molecules CD11b, CD16 and CD64 (neutrophils), CD154 (T lymphocytes), CD40 (B lymphocytes), and to quantitatively analyze the Treg cell subpopulation.

Methods: 49 elderlies (≥60 years) and 49 adults (≤35 years) were studied. Flow cytometry was used to analyze the expression of surface molecules and the subpopulation of Treg cells, and the results between the groups were compared statistically by the t-test.

Results: There was a decreased significance in the expression of CD11b and CD40 in the elderly.

Conclusion: Decreased CD11b expression can result in susceptibility to infectious diseases, and impairment of phagocytic capacity. Decreased CD40 expression can result in a decline in B lymphocyte activation. The other molecule studied presented alterations not significant, but compatible with the immunological changes in aging.

Keywords: CD11b; CD40; Immunosenescence; aging; lymphocytes; neutrophils..

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
B-Lymphocytes / immunology
B-Lymphocytes / metabolism
Biomarkers / metabolism
Brazil
CD11b Antigen / immunology
CD11b Antigen / metabolism*
CD40 Antigens / immunology
CD40 Antigens / metabolism*
CD40 Ligand / immunology
CD40 Ligand / metabolism
Cellular Senescence*
Cross-Sectional Studies
Down-Regulation
Female
Flow Cytometry
GPI-Linked Proteins / immunology
GPI-Linked Proteins / metabolism
Humans
Immunophenotyping / methods
Immunosenescence*
Lymphocytes / immunology
Lymphocytes / metabolism*
Male
Middle Aged
Neutrophils / immunology
Neutrophils / metabolism*
Receptors, IgG / immunology
Receptors, IgG / metabolism
T-Lymphocytes, Regulatory / immunology
T-Lymphocytes, Regulatory / metabolism
Young Adult

Substances

Biomarkers
CD11b Antigen
CD40 Antigens
FCGR3B protein, human
GPI-Linked Proteins
ITGAM protein, human
Receptors, IgG
CD40 Ligand