In this study, a novel helix acoustic resonator is proposed to realize acoustic energy harvesting (AEH). Compared with the traditional acoustic resonators, the proposed structure occupies a small volume and is suitable for the low frequency range. At a specific incident frequency, the mechanical component of the AEH device can be intensely excited and the bonded piezoelectric patch is utilized to convert the strain energy into electrical energy. Analytical studies are carried out to disclose the acoustic resonant system properties. Meanwhile, the pure acoustic and coupled vibro-acoustic properties of the proposed device are analyzed via the finite element method. The major part of the AEH device is fabricated via 3D printing for experimental study, which is favored for rapid prototyping. At acoustic resonance frequency 175 Hz, 100 dB sound pressure level excitation working condition, the measured experimental data show that the harvested power can be up to 7.3 μW.