Lateral stress profile and fluorescent lipid probes. FRET pair of probes that introduces minimal distortions into lipid packing

D S Tretiakova; A S Alekseeva; T R Galimzyanov; A M Boldyrev; A Yu Chernyadyev; Yu A Ermakov; O V Batishchev; E L Vodovozova; I A Boldyrev

doi:10.1016/j.bbamem.2018.05.020

Lateral stress profile and fluorescent lipid probes. FRET pair of probes that introduces minimal distortions into lipid packing

Biochim Biophys Acta Biomembr. 2018 Nov;1860(11):2337-2347. doi: 10.1016/j.bbamem.2018.05.020. Epub 2018 Jun 1.

Authors

D S Tretiakova¹, A S Alekseeva¹, T R Galimzyanov², A M Boldyrev³, A Yu Chernyadyev⁴, Yu A Ermakov⁴, O V Batishchev², E L Vodovozova⁵, I A Boldyrev⁶

Affiliations

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia; National University of Science and Technology MISiS, Leninskiy prospekt 4, Moscow 119049, Russia.
² A. N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 31/4 Leninskii Prospekt, Moscow 119071, Russia; National University of Science and Technology MISiS, Leninskiy prospekt 4, Moscow 119049, Russia.
³ Odintsovo, Mozhaiskoe shosse 86/71, Moscow Region 143005, Russia.
⁴ A. N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 31/4 Leninskii Prospekt, Moscow 119071, Russia.
⁵ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.
⁶ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia; Pirogov Russian National Research Medical University, 1 Ostrovityanov Street, Moscow 117997, Russia; National University of Science and Technology MISiS, Leninskiy prospekt 4, Moscow 119049, Russia. Electronic address: ivan@lipids.ibch.ru.

PMID: 29864406
DOI: 10.1016/j.bbamem.2018.05.020

Abstract

The modern theory of lipid membrane structure incorporates the concept of lateral stress profile. The latter represents the forces that act on any solute inside the membrane. We used this concept to propose two lipid probes that introduce minimal distortions into the lipid bilayer packing: the surface pressure isotherms and volt-potentials of the pure and mixed (probe-containing) lipid monolayers are equal. The probes represent a FRET pair. They are applicable in lipid transfer and vesicle fusion experiments.

Keywords: FRET; Fluorescent lipid probes; Lateral stress profile; Phospholipid bilayer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Boron Compounds / chemistry
Fluorescence Resonance Energy Transfer*
Fluorescent Dyes / chemistry*
Liposomes / chemistry*
Phosphatidylcholines / chemistry
Quantum Theory
Surface Properties

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Boron Compounds
Fluorescent Dyes
Liposomes
Phosphatidylcholines