Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite

Sabriye Yusan; Mokhlesur M Rahman; Nasir Mohamad; Tengku M Arrif; Ahmad Zubaidi A Latif; Mohd Aznan M A; Wan Sani B Wan Nik

doi:10.1155/2018/2687341

Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite

J Anal Methods Chem. 2018 May 10:2018:2687341. doi: 10.1155/2018/2687341. eCollection 2018.

Authors

Sabriye Yusan^{1

2}, Mokhlesur M Rahman³, Nasir Mohamad³, Tengku M Arrif³, Ahmad Zubaidi A Latif⁴, Mohd Aznan M A⁵, Wan Sani B Wan Nik⁶

Affiliations

¹ Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia.
² Institute of Nuclear Science, Ege University, Bornova, 35100 Izmir, Turkey.
³ Institute for Community Development & Quality of Life (i-CODE), Universiti Sultan Zainal Abidin, 21300 Kuala Nerus, Terengganu, Malaysia.
⁴ Faculty of Medicine, Universiti Sultan Zainal Abidin, 21300 Kuala Nerus, Terengganu, Malaysia.
⁵ Faculty of Medicine, International Islamic University Malaysia, 25200 Kuantan, Malaysia.
⁶ School of Ocean Engineering, Universiti Malaysia Terengganu, 21300 Kuala Nerus, Terengganu, Malaysia.

Abstract

A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 × 10^-5 to 2 × 10^-3 M. Under optimal conditions, the biosensor exhibits high sensitivity (0.34 µA·mM^-1), low detection limit (1 × 10^-4 M), high affinity to glucose (K_m = 0.02 mM), and also good reproducibility (R.S.D. 2.8%, n=10) and a stability of about ten days when stored dry at +4°C. Besides, the effects of pH value, scan rate, mediator effects on the glucose current, and electroactive interference of the biosensor were also discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.