Interaction of anions with lipid cubic phase membranes, an electrochemical impedance study

Mohammad Yaser Khani Meynaq; Britta Lindholm-Sethson; Solomon Tesfalidet

doi:10.1016/j.jcis.2018.05.071

Interaction of anions with lipid cubic phase membranes, an electrochemical impedance study

J Colloid Interface Sci. 2018 Oct 15:528:263-270. doi: 10.1016/j.jcis.2018.05.071. Epub 2018 May 29.

Authors

Mohammad Yaser Khani Meynaq¹, Britta Lindholm-Sethson¹, Solomon Tesfalidet²

Affiliations

¹ Department of Chemistry, Umeå University, SE 90187 Umeå, Sweden.
² Department of Chemistry, Umeå University, SE 90187 Umeå, Sweden. Electronic address: solomon.tesfalidet@umu.se.

PMID: 29859451
DOI: 10.1016/j.jcis.2018.05.071

Abstract

Hypothesis: Electrochemical impedance spectroscopy is useful to monitor anionic interactions with a Lipid Cubic Phase, as previously demonstrated for cationic interaction (Khani Meynaq et al., 2016). It was expected that the smaller hydrophilic anions, acetate and chloride, would interact differently than the large tryptophan anion with its hydrophobic tail.

Experiment: The impedance measurements enabled estimation of resistances and capacitances of a freestanding lipid cubic phase membrane at exposure to 4 and 40 mM solutions of NaCl, NaOAc and NaTrp. Small-angle X-ray scattering was used for cubic phase identification and to track structural changes within the cubic phase when exposed to the different electrolytes.

Findings: The membrane resistance increases at exposure to the electrolytes in the order Cl^- < OAc^- < Trp^-. The membrane resistance decreases with time at exposure to the hydrophilic anions and increases with time at Trp^- exposure. The membrane capacitances were lower for NaTrp compared to NaCl and NaOAc at the corresponding concentrations which is consistent with the results from SAXRD. It is concluded that Trp^- ions do not enter the aqueous channels of the cubic phase but are strongly adsorbed to the membrane/electrolyte interface leading to large alteration of the lipid phase structure and a high membrane resistance.

Keywords: Acetate; Anion; Capacitance; Chloride; Electrochemical impedance spectroscopy; Ionic interaction; Lipid cubic phase; Resistance; Small-angle X-ray diffraction; Tryphtophan.