Mouse Sperm Cryopreservation Using Cryoprotectant Containing l-Glutamine

Cold Spring Harb Protoc. 2018 Jun 1;2018(6). doi: 10.1101/pdb.prot094516.

Abstract

Efforts to advance sperm cryopreservation are ongoing and include modifications in the cryoprotective agents. The addition of l-glutamine maintains post-thaw motility and reduces plasma membrane damage to sperm.

MeSH terms

  • Animals
  • Cryopreservation*
  • Cryoprotective Agents / pharmacology*
  • Freezing
  • Glutamine / pharmacology*
  • Humans
  • Male
  • Mice
  • Semen Preservation
  • Spermatozoa / drug effects
  • Spermatozoa / physiology*

Substances

  • Cryoprotective Agents
  • Glutamine